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CIG@MGI Pipelines

The Collaborative and Intergrative Genomics (CIG) is a group in the McDonnell Genome Institute (MGI) at the Washington University School of Medicine (WUSM).

Overview

In this repo, we share genomics pipelines, tools, and tasks, mostly in the form of WDL. Corresponding Dockerfiles and scripts are at CIG Containters.

Repo Structure

Path Description
scripts helpers scripts for pipelines; these are not used in pipelines/tools
wdl/pipelines full start to end workflows that produce outputs from many tasks
wdl/tasks wrapped command line interfaces and scripts (must be incorporated into tools/pipelines)
wdl/tools stand alone workflows that combine a few tasks to produce outputs
wdl/structs data structures and types for pipelines, tools, and tasks

Building WDL Imports

WDLs have imports that bring in functionality from other WDL files. When running cromwell, a zip file of the imports is required. These WDL imports ZIP files are not included in the repo, but can be easily built with the build_imports script. From the main directory run the build_imports script giving the WDL file as the sole input. The output will be next to the WDL, named with the basename of the WDL file with the extension .wdl replaced with .imports.zip. Here is an example building the imports for wdl/tools/pacbio/align_output_cram.wdl:

$ cd cig-pipelines
$ bash ./scripts/build_imports wdl/tools/pacbio/align_output_cram.wdl 
WDL: wdl/tools/pacbio/align_output_cram.wdl
Archive:  align_output_cram.imports.zip
  Length      Date    Time    Name
---------  ---------- -----   ----
        0  2025-08-13 14:14   wdl/
        0  2025-08-13 14:14   wdl/structs/
      285  2025-08-13 14:14   wdl/structs/runenv.wdl
        0  2025-08-13 14:14   wdl/tasks/
        0  2025-08-13 14:14   wdl/tasks/pacbio/
     1568  2025-08-13 14:14   wdl/tasks/pacbio/pbmm2.wdl
---------                     -------
     1853                     6 files
Created imports file: wdl/tools/pacbio/align_output_cram.imports.zip

The tools and pipelines imports include the absolute paths of import WDL files. The tasks imports, which are typically limited, include the relative path of the imports. Pipeline and tool WDLs can use task WDLs, and task WDLs should only use other task WDLs.

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