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README.md
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+# TACAlgorithm
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+Computer Vision ImageJ Macro to label gold particles in immunogold labeled EM images
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+
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+1. Applies a fixed parameter gray-level threshold to the image.
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+2. Remaining binary components are filtered between a predetermined minimum and maximum area parameter.
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+ - These parameters are chosen based on the known sizes of gold particle contained in the image (e.g., 6 and 12 nm).
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+3. A circularity threshold is applied to each component to validate the common roundness of colloidal gold particles.
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