Skip to content

Commit 42f8426

Browse files
authored
Create README.md
1 parent 2ce571d commit 42f8426

File tree

1 file changed

+7
-0
lines changed

1 file changed

+7
-0
lines changed

README.md

Lines changed: 7 additions & 0 deletions
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,7 @@
1+
# TACAlgorithm
2+
Computer Vision ImageJ Macro to label gold particles in immunogold labeled EM images
3+
4+
1. Applies a fixed parameter gray-level threshold to the image.
5+
2. Remaining binary components are filtered between a predetermined minimum and maximum area parameter.
6+
- These parameters are chosen based on the known sizes of gold particle contained in the image (e.g., 6 and 12 nm).
7+
3. A circularity threshold is applied to each component to validate the common roundness of colloidal gold particles.

0 commit comments

Comments
 (0)