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2+
3+ run("8-bit");
4+ run("Set Measurements...", "centroid redirect=None decimal=3");
5+ run("Set Scale...", "distance=888 known=1 pixel=1 unit=nm");
6+ setThreshold(0, 92);
7+ setOption("BlackBackground", false);
8+ call("ij.plugin.filter.ParticleAnalyzer.setFontSize", 20);
9+ run("Analyze Particles...", "size=0.00009-0.0002 circularity=0.75-1.00 show=[Overlay Outlines] clear include add");
10+ resetThreshold();
11+ setTool("point");
12+ run("Point Tool...", "type=Circle color=Cyan size=[Extra Large] add label");
13+
14+
15+
16+ run("Undo");
17+ run("Set Measurements...", "centroid redirect=None decimal=3");
18+ run("Set Scale...", "distance=888 known=1 pixel=1 unit=nm");
19+ setTool("point");
20+ run("Point Tool...", "type=Circle color=Cyan size=[Extra Large] add label");
21+ waitForUser("Please fix any gold particles and then continue");
22+ roiManager("Deselect");
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