Hi Dr. Feng, when detecting minor SNVs, the program runs but does show a message of "igda_pipe_detect_ont: line 114: [: missing `]'". I looked into line 114 of igda_pipe_detect_ont, and it calls a $auto_par variable that is set to $OPTARG. Do you know what this error message is saying? I thought I inputted all required variables, and the program seems to be running smoothly, but I wanted to make sure. Thank you so much!