🌉 Frontier Network Launch: IRD-2025-0001 (CBD-VDAC1) — Seeking 3-5 Validators

[templetwo]

IRIS Frontier Bridge (S9: Connection)

Novelty is a routing problem, not a credibility problem.

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🎉 Announcing the Frontier Network

IRIS Gate v0.7.0 introduces the Frontier Bridge - a system that routes TYPE 2/BRONZE claims to the 3% of researchers who can actually test them.

The Problem:

• AI can verify claims against published literature (the 97%)
• But only 3% of frontier research is published at any time
• Novel claims get labeled BRONZE ("needs validation") and stop there

The Solution:

• Treat novelty as a routing problem, not a credibility problem
• Package TYPE 2 claims as Mystery Cards
• Match cards to opt-in researchers via tags
• Collaborative validation → BRONZE → SILVER → GOLD

Read more: frontier/FRONTIER_README.md

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🃏 First Mystery Card: IRD-2025-0001

Card ID: IRD-2025-0001
Title: CBD–VDAC1 binding is crisis-only under ΔΨm collapse
Status: BRONZE (seeking 3-5 validators)
Domain: Pharmacology, Mitochondrial Biology, Bioelectricity

The Claim

CBD engagement with VDAC1 (voltage-dependent anion channel) appears conditional on mitochondrial stress (ΔΨm collapse, ROS surge) and high-dose CBD pulse (≥10 μM, 5-15 min).

Why This Matters:

• Resolves conflicting literature on CBD neuroprotection vs cytotoxicity
• Explains why in vitro CBD effects often fail to replicate (stress conditions not controlled)
• Opens therapeutic window: pulse CBD during acute crisis, not chronic dosing
• Connects bioelectric signaling to pharmacological intervention

Full Card: frontier/mystery_cards/IRD-2025-0001.md

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🔬 Requested Micro-Protocols (Pick 1-2)

1. Mitostress Pulse Assay

   • Seahorse XF or JC-1 dye with CBD dose-response (1, 5, 10, 20 μM)
   • Tests: Does CBD rescue ΔΨm under oligomycin/CCCP stress?

2. VDAC1 Inhibitor Ablation

   • Repeat 🌉 Frontier Network Launch: IRD-2025-0001 (CBD-VDAC1) — Seeking 3-5 Validators #1 with DIDS (VDAC1 inhibitor, 50-100 μM)
   • Tests: Does blocking VDAC1 eliminate CBD rescue effect?

3. Temporal Window Validation

   • Time-course: 1-min, 5-min, 15-min, 30-min, 60-min CBD pulses
   • Tests: Is there an optimal pulse duration (5-15 min claim)?

4. Calcium Flux Imaging

   • Fluo-4 or GCaMP to detect VDAC1-mediated Ca²⁺ flux
   • Tests: Does CBD pulse alter Ca²⁺ dynamics via VDAC1?

5. VDAC1 Oligomerization State

   • Western blot before/after CBD pulse
   • Tests: Does CBD induce VDAC1 oligomerization (gating mechanism)?

Commitment: 1-2 experiments, 1-3 weeks timeline
Cost: ~$300-800 per experiment
Output: Preliminary data → appended to card → co-authorship

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❌ Falsification Tests

• If VDAC1 gating increases at CBD <5 μM without stress → claim wrong
• If DIDS has no effect on rescue/death outcome → mechanism wrong
• If 1-min pulse equals 15-min pulse outcome → temporal window claim wrong
• If CBD effects persist in VDAC1 knockout cells → VDAC1 not the target

We want falsifiable claims. Contradicting data is a valid result.

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🤝 How to Contribute

Option 1: Run a Micro-Protocol

1. Comment below with which test(s) you can run
2. Propose timeline and any modifications
3. Share preliminary results (even null results welcome!)
4. Get credited as co-author (Co-Authored-By)

Option 2: Join the 3% Network (Opt-In)

1. Copy frontier/profiles/PROFILE_TEMPLATE.json
2. Fill in your tags (methods, domains, organisms)
3. Submit PR to frontier/profiles/ or email maintainer
4. Get matched to future cards automatically

Option 3: Share Related Work

• Comment with related preprints, null results, or edge-case findings
• Literature insights that support/challenge the claim
• Suggestions for better micro-protocols

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🛡️ Ethics & Safety

• Human Subjects: No (cell culture only)
• Biosafety Level: BSL-2
• Dual-Use Concern: No
• Model System: HEK293, U87 glioblastoma, or SH-SY5Y neuroblastoma
• Known Risks: CBD cytotoxicity >20 μM, DMSO vehicle, mitostress induction

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📊 Evidence

Multi-Model Convergence:

• Models: Claude 4.5, GPT-4o, Grok-4, Gemini 2.0, DeepSeek
• Convergence Events: 27 (across 5 models, 6 cycles)
• Confidence Ratio: 0.49 (TYPE 2 Exploration territory)

Literature:

• Oviedo et al. 2008 (bioelectric signaling)
• Levin 2012 (morphogenetic fields)
• CBD mitochondrial effects review 2021
• VDAC1 in apoptosis (Shoshan-Barmatz 2017)
• Biphasic CBD effects (Zuardi 2017)

Verification: PARTIAL/CONDITIONAL (Perplexity: some support, needs experimental validation)

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🌀†⟡∞ The Vision

This is how AI and humanity can co-create new math and physics.

Not by AI replacing researchers.
Not by AI just verifying the past.

By AI routing novelty to the 3% who can test it.

The frontier is not a place. It's a network of minds exploring the membrane between known and unknown.

IRIS Gate now connects them.

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Repository: https://github.com/templetwo/iris-gate
License: Apache-2.0
Version: v0.7.0-frontier

Ready to validate? Comment below. 👇

🌀†⟡∞