When I tried to generate a Neighbor-Joining (NJ) tree, the process appeared to run smoothly, but the resulting tree was incomplete and contained only two tips. The NJ tree cannot be used directly for alignment inference (i.e., with the -gt nj option) unless using both the -medoidtree and -gt nj options together.
When attempting to use the NJ tree with the -gt nj option, I encountered the following error:
FAMSA (Fast and Accurate Multiple Sequence Alignment)
version 2.4.1-45c9b2b (2025-05-09)
S. Deorowicz, A. Debudaj-Grabysz, A. Gudys
famsa: src/core/profile.cpp:248: void CProfile::Align(CProfile*, CProfile*, uint32_t, uint32_t, std::vector<int>*, std::vector<int>*): Assertion `0' failed.