Unable to read .mzML files

[StSchulze]

I am using the most current pGlyco version (2019.01.01) which is supposed to support mzML files, but when I try to use an mzML file, I get the following error

[pParse] Path Invalid! datapath = C:\Users\Admin\Desktop\pGlyco2\20150708_QE3_UPLC8_DBJ_SA_Hela_39frac_Trypsin_18.mzML

[pParse] Task abort!
[pParse] pParse has encountered a problem and will exit. Retry with correct parameters.

The path is correct/the file is located at the given location (it was selected in the GUI by browsing), so I'm not sure what that error means.
The corresponding .raw file works perfectly fine.
I have converted it using ProteoWizard 3.0.19046

The full report is attached.
pGlyco_task_2019-04-29-18-11-46.log

Thanks for any help on this.

[jalew188]

@StSchulze please check https://github.com/pFindStudio/pParse_pXtract/blob/master/README.md to see how pGlyco supports mzML.

[StSchulze]

@jalew188 Thanks for the link. I converted the .raw to .mzML again with msconvert, using the described settings. Unfortunately, it fails with the same error as before, so it doesn't seem to solve the problem.

I have added a screenshot of the msconvert settings in case I have missed something.

[jalew188]

Hi, the problem is not from ProteoWizard, it seems that pParse does not find the file C:\Users\Admin\Desktop\pGlyco2\20150708_QE3_UPLC8_DBJ_SA_Hela_39frac_Trypsin_18.mzML. I will try mzML files in these days.

[StSchulze]

Yeah, that's what I initially thought as well.
As mentioned above the path is correct (and the file has been selected through browsing with the GUI).
Thanks for having a look into this.