No Cluster 0

[esbrady]

Hi Ferchaure,

I was just wondering, in instances where there is a cluster 1 but no cluster 0 (see below), does this mean that the detected units that would usually fall into cluster 0 have all grouped together to form cluster 1 instead? Is it likely that this cluster represents MUA/noise rather than SUA?

Many thanks,
Erica

[ferchaure]

Hi Erica,

Technically cluster 0 are the spikes thar are too far away from the known templates and usually (not a real criterion) cluster 1 is the main clusters and MUA. In your case, everything is really similar to the single template found.

In my opinion:
-Try with a positive threshold I saw a few positive peaks

• That is MUA (or maybe just noise with a peak, try increasing a the detection threshold) and oddly fast for a neuron

[esbrady]

Thank you, Ferchaure! This is very helpful.

Other than noticing a large number of spikes that break the refractory limit, what are some other ways you can distinguish MUA from SUA in the wave_clus output?

[ferchaure]

It's complicated a few MU criteria could be:

• The smallest waveform not far away from the peak... far away neurons look the same (specially when the SNR is low).
• Different levels of noise in some samples that gives you the idea that there are more than one waveform there.