Understanding how to caluclate splice site strength

[adi8897]

Hi I read your paper several times
Im trying to understand if I can calculate splice site strength from rna seq I have (in the form of bam files)
I have coordinates of spefsific splice sites I want to detect
but I'm a bit lost.
Do I have to have the vcf file?
Sorry if that a silly question

[SilverNebula]

VCF files are not required. Using our method, the usage of a splice site is calculated based on the proportion of samples (i.e., the BAM files in this case) in which the site can be detected.

[adi8897]

Can you please detail a bit more on how it can be done
i have a bed file and fasta files with the sequences I want to examine but how is it done?
I dont see any detailes on splice site strength in the git