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Update README.md
updating readme for V1.1.0
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# Pipeline in 6 steps: <br/>
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### 1. Downsampling of reads to a designated coverage using ```Filtlong```
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### 1. Downsampling of reads to a designated coverage using [Filtlong](https://github.com/rrwick/Filtlong)
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##### &nbsp; &nbsp; -default is set to 100X (-x); which provided better assemblies compared to the typical 30-50X
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### 2. _Optional_: Polishing of downsampled reads with the paired-end illumina reads using ```Ratatosk correct```
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### 2. _Optional_: Polishing of downsampled reads with the paired-end illumina reads using [Ratatosk](https://github.com/DecodeGenetics/Ratatosk)
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##### &nbsp; &nbsp; -uses a baseline quality score (-Q) of 90 and therefore assumes mildly recent ONT data (e.g. R10 or high-accuracy basecalling)
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### 3. Genome Assembly
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#### 3.a. Assembly with```Flye```
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#### 3.a. Assembly with [Flye](https://github.com/mikolmogorov/Flye)
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##### &nbsp; &nbsp; -removed the hard coded maximium value for the minimum overlap threshold (previously 10kb)
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##### &nbsp; &nbsp; -by default the minimum overlap value is automatically provided as the read N90 after polishing
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#### 3.b. Assembly with ```Hifiasm```
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#### 3.b. Assembly with [Hifiasm](https://github.com/chhylp123/hifiasm)
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##### &nbsp; &nbsp; -if Hifi reads are provided: uses the ```--ul``` option, with both polished ONT and Hifi reads
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##### &nbsp; &nbsp; -without Hifi: uses the ```--ont``` option, with only the polished ONT reads
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### 4. 'Patch' the Flye assembly (target) using the the Hifiasm assembly (query) with ```Ragtag patch```
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### 4. 'Patch' the Flye assembly (target) using the the Hifiasm assembly (query) with [Ragtag](https://github.com/malonge/RagTag/wiki/patch)
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##### &nbsp; &nbsp; -uses a minimum unique alignment length (-f) of 25000 to be conservative during patching
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### 5. _Optional_: Polishing of assembly with PacBio Hifi and paired-end illumina reads using ```NextPolish2```
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### 6. Filtering (minimum contig length 10kb), reordering and renaming using ```Seqkit``` and ```awk```
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### 5. _Optional_: Polishing of assembly with PacBio Hifi and paired-end illumina reads using [NextPolish2](https://github.com/Nextomics/NextPolish2)
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### 6. Filtering (minimum contig length 10kb), reordering and renaming using [Seqkit](https://bioinf.shenwei.me/seqkit) and ```awk```
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### 7. Comprehensive evaluation of all assemblies using [PAQman](https://github.com/SAMtoBAM/PAQman)
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## Schematic
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<p align="center" >
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<img src="https://github.com/SAMtoBAM/fusemblr/blob/main/figures/fusemblr_schematic.png" width=70%>
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<img src="https://github.com/SAMtoBAM/fusemblr/blob/main/figures/fusemblr_schematic.updated.png" width=70%>
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</p>
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Following assembly it is recommended that you run [PAQman](https://github.com/SAMtoBAM/PAQman) on your resulting assembly to comprehensively check the quality <br/>
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It is recommended to feed your resulting assembly to PAQman alongside the 1.filtlong/*.fz.gz set of reads <br/>
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This can also help you compare any assemblies you have to check for the best.
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