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Merge pull request #143 from PMCC-BioinformaticsCore/zeroinflation_xl
update links in vignettes/macpie.Rmd
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vignettes/macpie.Rmd

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@@ -31,8 +31,8 @@ MAC-seq is a cost-effective, high-throughput transcriptomic platform, developed
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In this vignette we cover the basic functionality of <b>macpie</b>, from input, quality control to transcriptional and screen-related analyses. For more in-depth workflows, please refer to other vignettes:
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- [Quality control](quality_control.html)
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- [Transcriptional analysis](transcriptional_analysis.html)
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- [Compound screening](compound_screening.html)
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- [Transcriptional analysis](transcriptional_analyses.html)
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- [Compound screening](high_throughput_screens.html)
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- [Cross-platform compatibility](cross_platform_compatibility.html)
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### 1. Metadata import and QC
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- use **plot_mds** to check sample grouping (umap/pca is also available using Seurat's functions)
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- use **compute_qc_metrics**, **plot_qc_metrics_heatmap**, and **plot_distance** to check sample variability and outliers
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- use **plot_rle** to check any row/column/plate effects and compare normalization methods
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- more detailed methods avaailable in vignette [Quality control](articles/quality_control.html)
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- more detailed methods available in vignette [Quality control](articles/quality_control.html)
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#### 2.1 Import data to tidySeurat object
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gene expression or pathway enrichment
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- use **compute_multi_screen_profile** to find perturbations similar to your target profile or
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a known gene set
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- more detailed methods avaailable in vignette [Compound screening](articles/compound_screening.html)
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- more detailed methods available in vignette [Compound screening](articles/high_throughput_screens.html)
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#### 4.1. UMAP clustering based on DE genes
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